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- Title
Anti-Melanogenic Effects of Ethanol Extracts of the Leaves and Roots of Patrinia villosa (Thunb.) Juss through Their Inhibition of CREB and Induction of ERK and Autophagy.
- Authors
Jeong, Deok; Park, Sang Hee; Kim, Min-Ha; Lee, Sarah; Cho, Yoon Kyung; Kim, You Ah; Park, Byoung Jun; Lee, Jongsung; Kang, Hakhee; Cho, Jae Youl; Capasso, Raffaele; Mannelli, Lorenzo Di Cesare
- Abstract
Patrinia villosa (Thunb.) Juss is a traditional herb commonly used in East Asia including Korea, Japan, and China. It has been administered to reduce and treat inflammation in Donguibogam, Korea. The mechanism for its anti-inflammatory effects has already been reported. In this study, we confirmed the efficacy of Patrinia villosa (Thunb.) Juss ethanol extract (Pv-EE) for inducing autophagy and investigate its anti-melanogenic properties. Melanin secretion and content were investigated using cells from the melanoma cell line B16F10. Pv-EE inhibited melanin in melanogenesis induced by α-melanocyte-stimulating hormone (α-MSH). The mechanism of inhibition of Pv-EE was confirmed by suppressing the mRNA of microphthalmia-associated transcription factor (MITF), decreasing the phosphorylation level of CREB, and increasing the phosphorylation of ERK. Finally, it was confirmed that Pv-EE induces autophagy through the autophagy markers LC3B and p62, and that the anti-melanogenic effect of Pv-EE is inhibited by the autophagy inhibitor 3-methyl adenine (3-MA). These results suggest that Pv-EE may be used as a skin protectant due to its anti-melanin properties including autophagy.
- Subjects
SOUTH Korea; EAST Asia; AUTOPHAGY; MICROPHTHALMIA-associated transcription factor; MESSENGER RNA; ETHANOL
- Publication
Molecules, 2020, Vol 25, Issue 22, p5375
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules25225375