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- Title
Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set.
- Authors
Csank, Tomáš; Bhide, Katarína; Bencúrová, Elena; Dolinská, Saskia; Drzewnioková, Petra; Major, Peter; Korytár, Ľuboš; Bocková, Eva; Bhide, Mangesh; Pistl, Juraj
- Abstract
West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by sequencing. WNV was detected in swab and brain samples, whereas one brain sample was positive for tick-borne encephalitis virus (TBEV). The WNV sequences clustered with lineages 1 and 2. These results confirm the circulation of WNV in birds in Slovakia and emphasize the risk of infection of humans and horses.
- Subjects
SLOVAKIA; WEST Nile fever diagnosis; TICK-borne encephalitis viruses; BIRD diseases; PUBLIC health
- Publication
Archives of Virology, 2016, Vol 161, Issue 6, p1679
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-016-2828-5