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- Title
Differential Expression of Bcl-2 in the Cochlea and Auditory Cortex of a Mouse Model of Age-Related Hearing Loss.
- Authors
Huang, Qiuhong; Xiong, Hao; Yang, Haidi; Ou, Yongkang; Zhang, Zhigang; Chen, Suijun; Ye, Yongyi; Zheng, Yiqing
- Abstract
Bcl-2, the first gene shown to be involved in apoptosis, is a potent regulator of cell survival and known to have protective effects against a variety of age-related diseases. However, the possible relationship between hearing and Bcl-2 expression in the cochlea or auditory cortex of C57BL/6 mice, a mouse model of age-related hearing loss, is still unknown. Using RT-PCR, immunohistochemistry, and Western blot analysis, our results show that Bcl-2 is strongly expressed in the inner hair cells and spiral ganglion neurons of young mice. In addition, moderate Bcl-2 expression is also detected in the outer hair cells and in the neurons of the auditory cortex. A significant reduction of Bcl-2 expression in the cochlea or auditory cortex is also associated with elevated hearing thresholds and hair cell loss during aging. The expression pattern of Bcl-2 in the peripheral and central auditory systems suggests that Bcl-2 may play an important role in auditory function serving as a protective molecule against age-related hearing loss.
- Subjects
BCL-2 genes; HEARING; AUDITORY cortex; BRAIN stem physiology; PROTEIN metabolism; ACOUSTIC nerve; AGING; ANIMAL experimentation; ANIMALS; AUDITORY evoked response; BIOLOGICAL models; COCHLEA; HAIR cells; HEARING levels; IMMUNOHISTOCHEMISTRY; MICE; NEURONS; POLYMERASE chain reaction; PRESBYCUSIS; PROTEINS; WESTERN immunoblotting; REVERSE transcriptase polymerase chain reaction
- Publication
Audiology & Neurotology, 2017, Vol 21, Issue 5, p326
- ISSN
1420-3030
- Publication type
journal article
- DOI
10.1159/000450937