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- Title
OPTIMIZATION AND PRODUCTION OF AMYLASE FROM ASPERGILLUS NIGER PBF21 ISOLATED FROM MARINE BIOFILM.
- Authors
Udayadevi, M.; Harikrishnan, S.; Sivasubramani, K.; Sureshkumar, P.
- Abstract
The aim of the present study was to isolate a marine fungus with promising amylase production potential from the biofilms. In the present study, biofilm samples were collected from surfaces of boats, concrete structures and mangrove trees submerged during high tide and exposed during low tide in the Parangipettai area of Tamil Nadu, India and were used for the screening and isolation of a promising marine fungus for amylase production. A total of 82 fungi were isolated. Among them, the isolate PBF21 isolated from the boat biofilm showed highest amylase activity was identified as Aspergillus niger and it was further selected for optimization, mass scale cultivation and amylase production. The ideal conditions for the maximum biomass and amylase production of the potential strain A. niger were 3% inoculum concentration, 96 hrs of incubation period, 50 rpm agitation, pH 6, 35°C, 1% salinity, 1.5% starch as the carbon source and 0.6% peptone as the nitrogen source. Regarding the cheaper substrates tested for biomass and amylase production, sago industrial effluent (at 5%) gave 5.0 g/L and 913 U/ml/min. whereas, it was 4.6 g/L and 852 U/ml/min. with 6% sugarcane molasses. Mass scale cultivation in shake flask with the above conditions gave a maximum biomass of 8.6 g/L with the amylase activity of 1797 U/ml/min. with the standard substrates whereas it was 5.2 g/L with the amylase activity of 1021 U/ml/min. when using sago industrial effluent (SIE) as a cheaper substrate.
- Publication
Biochemical & Cellular Archives, 2023, Vol 23, Issue 1, p241
- ISSN
0972-5075
- Publication type
Article
- DOI
10.51470/bca.2023.23.1.241