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- Title
Associations Among Bovine Melatonin Receptor 1A and Dopamine D2 Receptor Gene Mutations, Hair Coat Scores, and Calving Rates in Beef Cattle.
- Authors
Meyer, L. R.; Powell, J. G.; Kutz, B. R.; Looper, M. L.; Rosenkrans Jr., C. F.
- Abstract
Hair coat scores of beef cattle have been associated with productivity traits. Our objective was to determine relationships among single nucleotide polymorphisms (SNP) in bovine melatonin receptor 1A (MTNR1A), dopamine D2 receptor (DRD2), and cattle hair coat shedding and productivity traits. Genomic DNA was isolated from buffy coat samples of fall-calving Angus-based crossbred cows (n = 104). Purified DNA was amplified using specific primers for an 845-base segment of MTNR1A [forward (5'-TAGTTAACGATGGGTGGAGC- 3') and reverse (5'-AAATGAGTAAGGCTTGGAGC- 3')], and a 793-base segment of DRD2 [forward (5'-TATAGCCCCATTCCTGATTC-3') and reverse (5'-CCCATGCTCTACAACACACG-3')]. Amplicons were forward strand sequenced using Eurofins SimpleSeq (Louisville, KY); sequences were compared using MEGA version 6 for polymorphism identification and genotype assignment. Three years (2012, 2013, and 2014) of production data were used to determine associations between genotypes, and calving rate and hair shedding score. Hair coat scores were determined each year over 4 mo (April, May, June, and July). Relationships were determined using mixed model ANOVA with main effects of year, month, and genotype. For MTNR1A, five transition SNP (minor allele frequency) were identified [A541G (4.8%), G575A (4.8%), A583G (46.2%), T679C (4.8%), and C721T (4.8%)], and one transition SNP was identified for DRD2 [A534G (46.6%)]. Homozygous cows at A583G had increased (P < 0.05) calving percentage when compared with heterozygous cows (97, 99, and 88%, respectively, for AA, GG, AG). At A583G, AA cows shed winter hair coats earlier and had lower (P < 0.05) hair coat scores than AG and GG cows in May. Genotype at DRD2 (A534G) tended (P = 0.09) to affect hair coat score, but did not affect (P > 0.7) calving rates. Results suggest that MTNR1A mutation A583G may be a useful tool for selecting replacement cattle that will have increased calf crops.
- Subjects
BEEF cattle breeding; DOPAMINE; CATTLE parturition
- Publication
Journal of Animal Science, 2017, Vol 95, p45
- ISSN
0021-8812
- Publication type
Abstract
- DOI
10.2527/ssasas2017.090