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- Title
Thermophilic bacterial DNA polymerases with reverse-transcriptase activity.
- Authors
Shandilya, Harini; Griffiths, Kate; Flynn, Elizabeth K.; Astatke, Mekbib; Shih, Po-Jen; Lee, Jun E.; Gerard, Gary F.; Gibbs, Moreland D.; Bergquist, Peter L.
- Abstract
Conserved motifs found in known bacterial polI DNA polymerase sequences were identified, and degenerate PCR primers were designed for PCR amplification of an internal portion of polI genes from all bacterial divisions. We describe here a method that has allowed the rapid identification and isolation of 13 polI genes from a diverse selection of thermophilic bacteria and report on the biochemical characteristics of nine of the purified recombinant enzymes. Several enzymes showed significant reverse-transcriptase activity in the presence of Mg2+, particularly the polymerases from Bacillus caldolyticus EA1, Caldibacillus cellovorans CompA.2, and Clostridium stercorarium.
- Subjects
DNA polymerases; TRANSFERASES; THERMOPHILIC bacteria; THERMOPHILIC microorganisms; REVERSE transcriptase; BIOCHEMISTRY
- Publication
Extremophiles, 2004, Vol 8, Issue 3, p243
- ISSN
1431-0651
- Publication type
Article
- DOI
10.1007/s00792-004-0384-5