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- Title
The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella.
- Authors
Lechtreck, Karl-Ferdinand; Johnson, Eric C.; Sakai, Tsuyoshi; Cochran, Deborah; Ballif, Bryan A.; Rush, John; Pazour, Gregory J.; Ikebe, Mitsuo; Witman, George B.
- Abstract
In humans, seven evolutionarily conserved genes that cause the cilia-related disorder Bardet-Biedl syndrome (BBS) encode proteins that form a complex termed the BBSome. The function of the BBSome in the cilium is not well understood. We purified a BBSome-like complex from Chlamydomonas reinhardtii flagella and found that it contains at least BBS1, -4, -5, -7, and -8 and undergoes intraflagellar transport (IFT) in association with a subset of IFT particles. C. reinhardtii insertional mutants defective in BBS 1, -4, and -7 assemble motile, full-length flagella but lack the ability to phototax. In the bbs4 mutant, the assembly and transport of IFT particles are unaffected, but the flagella abnormally accumulate several signaling proteins that may disrupt phototaxis. We conclude that the BBSome is carried by IFT but is an adapter rather than an integral component of the IFT machinery. C. reinhardtii BBS4 may be required for the export of signaling proteins from the flagellum via IFT.
- Subjects
MICROBIAL proteins; CHLAMYDOMONAS reinhardtii; LAURENCE-Moon-Biedl syndrome; FLAGELLA (Microbiology); CILIA &; ciliary motion; PHOTOTAXIS; BIOLOGICAL transport
- Publication
Journal of Cell Biology, 2009, Vol 187, Issue 7, p1117
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200909183