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- Title
Puerarin Inhibits C-Reactive Protein Expression via Suppression of Nuclear Factor κB Activation in Lipopolysaccharide-Induced Peripheral Blood Mononuclear Cells of Patients with Stable Angina Pectoris.
- Authors
Xiangjun Yang; Wenzhi Hu; Qin Zhang; Yueying Wang; Lie Sun
- Abstract
Puerarin (4′-7-dihydroxy-8-β-d-glucosylisoflavone), the most abundant isoflavone-C-glucoside extracted from the root of the plant Pueraria lobata, has demonstrated anti-inflammatory activity in cellular models of inflammation. In this report, we examined the ability of puerarin to modulate C-reactive protein (CRP) expression and key molecules in the nuclear factor kappa B (NF-κB) pathway to determine its molecular target. The protein and mRNA levels of CRP were determined in lipopolysaccharide (LPS)-induced peripheral blood mononuclear cells of patients with unstable angina pectoris. Also, we detected the I-κBα phosphorylation and the p65NF-κB expression in peripheral blood mononuclear cells under our experimental condition. The results indicated that puerarin inhibited the expression of the protein and mRNA levels of CRP in LPS-induced peripheral blood mononuclear cells. Subsequently, we determined that the inhibition of CRP expression was because of a dose-dependent inhibition of phosphorylation and degradation of inhibitor kappaB(I-κB), which resulted in a reduction of p65NF-κB nuclear translocation. We conclude that puerarin acts as an anti-inflammatory agent by blocking NF-κB signalling, and may possibly be developed as a useful agent for the chemoprevention of atherosclerosis.
- Subjects
C-reactive protein; ENDOTOXINS; ANGINA pectoris; MESSENGER RNA; PHOSPHORYLATION
- Publication
Basic & Clinical Pharmacology & Toxicology, 2010, Vol 107, Issue 2, p637
- ISSN
1742-7835
- Publication type
Article
- DOI
10.1111/j.1742-7843.2010.00548.x