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- Title
Pantoprazole promotes BMSCs proliferation and osteoblastic differentiation via NO pathway.
- Authors
WEI Hongyan; TAN Boyu; CHEN Li; DENG Nan
- Abstract
Objective To investigate the effect of pantoprazole, a proton pump inhibitor, on the proliferation and osteoblastic differentiation of primary rat bone marrow-derived mesenchymal stem cells ( BMSCs), and to explore its possible mechanism. Methods Rat BMSCs were cultured in osteogenic differentiation medium, consisting of phenol red-free α-MEM plus 10% FBS (dextran-coated charcoal stripped, DCS) supplemented with 10 mmol/L β-glycerophosphate, 50mg/L ascorbic acid, and 10 nmol/ L dexamethasone, in order to induce the osteoblastic differentiation of BMSCs. Then, different concentrations of pantoprazole (0. 0140 µmol/L) were additioned into the medium, and its effect on BMSCs was observed. The cell proliferation was measured by MTT incorporation. The osteoblastic differentiation of BMSCs was assessed by the detection of cellular alkaline phosphatase (ALP) activity and calcium deposition. The output of nitric oxide (NO) was detected using a NO kit. Results In a concentration range (0. 1-10 µmol/L), pantoprazole increased MTT incorporation, ALP activity, and calcium deposition of BMSCs in a dose-dependent manner. The output of NO also increased moderately. Conclusion Pantoprazole, at the concentration of 0. 1-10 µmol/L, can promote proliferation and osteoblastic differentiation of primary BMSCs via NO pathway.
- Subjects
PROTON pump inhibitors; OSTEOBLASTS; MESENCHYMAL stem cells; PHARMACODYNAMICS; CELL differentiation; CELL proliferation; ALKALINE phosphatase; NITRIC oxide
- Publication
Chinese Journal of Osteoporosis, 2014, Vol 20, Issue 4, p375
- ISSN
1006-7108
- Publication type
Article
- DOI
10.3969/j.issn.1006-7108.2014.04.007