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- Title
4-Acetylantrocamol LT3, a New Ubiquinone from Antrodia cinnamomea, Inhibits Hepatocellular Carcinoma HepG2 Cell Growth by Targeting YAP/TAZ, mTOR, and WNT/β-Catenin Signaling.
- Authors
Chen, Yen-Lin; Yen, I-Chuan; Lin, Kuen-Tze; Lai, Feng-Yi; Lee, Shih-Yu
- Abstract
4-acetylantrocamol LT3 (4AALT3), a new ubiquinone from the mycelium of Antrodia cinnamomea (Polyporaceae), has been recently shown to possess anticancer activity. However, the detailed mechanisms of such action remain unclear. In this study, the molecular mechanisms of 4AALT3 on hepatocellular carcinoma cells (HCC) were investigated. Human hepatocellular carcinoma cell line HepG2 cells were treated with concentrations of 4AALT3. Cell viability, colony formation, and the underlying mechanisms were then analyzed by CCK-8, colony formation, qPCR, and Western blotting assays. We found that 4AALT3 significantly decreased cell viability and colony formation in a dose-dependent manner. Accordingly, 4AALT3 significantly decreased protein levels of cyclin B, E1, D1, and D3, thereby facilitating cell cycle arrest. In addition, 4AALT3 significantly suppressed the nuclear localization of Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ), mammalian target of rapamycin (mTOR), and WNT/ β -catenin signaling pathways, all of which are well-known signaling pathways that contribute to the malignant properties of HCC. These effects are associated with activation of 5′ AMP-activated protein kinase (AMPK) and autophagy. Our findings indicate that 4AALT3 exerts inhibitory effects on HepG2 cell growth via multiple signaling pathways and may be a potential agent for HCC therapy.
- Subjects
PROTEIN kinases; PROTEINS; FLOW cytometry; STATISTICS; WESTERN immunoblotting; ONE-way analysis of variance; FUNGI; CYTOSKELETAL proteins; UBIQUINONES; CELLULAR signal transduction; CELL survival; CELL cycle; GENE expression; CHALONES; DESCRIPTIVE statistics; TRANSFERASES; RESEARCH funding; CELL lines; EPITHELIAL cells; POLYMERASE chain reaction; CHROMATOGRAPHIC analysis; DATA analysis; DATA analysis software; HEPATOCELLULAR carcinoma; CHEMICAL inhibitors
- Publication
American Journal of Chinese Medicine, 2020, Vol 48, Issue 5, p1243
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X20500615