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- Title
Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers.
- Authors
Xiang-Shan Ji; Yong-Sheng Tian; Jing-Feng Yang; Peng-Fei Wu; Yun-Liang Jiang; Song-Lin Chen
- Abstract
Effective methods for induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. An effective protocol to induce half-smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm−2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 °C for 20–23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female-specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.
- Subjects
CYNOGLOSSUS; GYNOGENESIS; FISH spermatozoa; POLYMERASE chain reaction; HEREDITY
- Publication
Aquaculture Research, 2010, Vol 41, Issue 6, p913
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/j.1365-2109.2009.02373.x