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- Title
MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 but not MET amplification, especially in high-grade cases: clinical and pathological review of 30 myxofibrosarcoma cases.
- Authors
Ma, Shirong; Fan, Linni; Liu, Yixiong; Wang, Yingmei; Yu, Kangjie; Wang, Lifeng; Fang, Na; Liu, Fang; Guo, Shuangping; Wang, Zhe
- Abstract
Background: Myxofibrosarcoma (MFS) is one of the most common soft tissue sarcomas. Previous studies have shown that MET protein overexpressed in MFS patients and can serve as a prognostic factor. The reasons for MET protein overexpression include amplification of the MET gene, which is located on chromosome 7q. Triggered by an index case harboring chromosome 7 polysomy rather than MET gene amplification in myxofibrosarcoma, we investigated chromosome 7 polysomy in more cases. Methods: Immunohistochemistry and fluorescence in situ hybridization (FISH) were performed in 30 MFS cases (including 2 epithelioid variant) to detect the expression of MET protein and gene status. Results: MET was overexpressed in 14 cases out of 30, while thirteen cases were in higher FNCLCC grades (Grade 2–3). FISH showed that 11 cases having 3 signals on average of Met and more than 3 signals (Mean: 4.6) of centromere 7q (CEP7q). The MET/CEP7 ratio was about 0.65 on average, suggesting that chromosome 7 polysomy, rather than Met gene amplification, leading to the overexpression of MET protein in MFS. MET overexpression and chromosome 7 polysomy are positively correlated with higher Ki-67 index and higher grade and might have a high risk of local recurrence and metastasis. Conclusions: It might reveals another explain of MET overexpression in myxofibrosarcoma, providing a clue for the therapy of MFS.
- Subjects
GENETIC overexpression; CHROMOSOMES; PROTEIN expression; IMMUNOHISTOCHEMISTRY; FLUORESCENCE in situ hybridization; GENE amplification; SARCOMA
- Publication
Diagnostic Pathology, 2018, Vol 13, Issue 1, pN.PAG
- ISSN
1746-1596
- Publication type
Article
- DOI
10.1186/s13000-018-0733-9