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- Title
A multicompartmental Model of In Vivo Adipose Tissue Glycerol Kinetics and Capillary Permeability in Lean and Obese Humans.
- Authors
Coppack, Simon W.; Chinkes, David L.; Miles, John M.; Patterson, Bruce W.; Klein, Samuel
- Abstract
Lipolysis of adipose tissue triglycerides releases glycerol. Twenty-four volunteers, of whom 6 were obese and 13 were women, received a primed-constant infusion of ²H5-glycerol for 120 min during postabsorptive steady-state conditions. Arterial, abdominal venous, and interstitial (microdialysis) samples were taken, and a four-compartment model was applied to assess subcutaneous abdominal adipose tissue glycerol kinetics. Adipose tissue blood flow was measured using 133Xe washout. Venous glycerol concentrations (median 230 µmol/l [interquartile range 210-268]) were consistently greater than those of arterial blood (69.1 µmol/l [56.5-85.5]), while glycerol isotopic enrichments (tracer-to-tracee ratio) were greater in arterial blood (8.34% [7.4410.1]) than venous blood (2.34% [1.71-2.69], P < 0.01). Microdialysate glycerol enrichment was 1.44% (1.11-1.79), indicating incomplete permeability of glycerol between capillary blood and interstitium. Calculated interstitial glycerol concentrations were between 270 µmol/l (256-350) and 332 µmol/l (281-371) (examining different boundary conditions). The calculated capillary diffusion capacity (ps) was between 2.21 ml ⋅ 100 g tissue-1 ⋅ min-1 (1.31-3.13) and 3.09 ml ⋅ 100 g tissue-a ⋅ min-1 (1.52-4.90) and correlated inversely with adiposity (R[sub s] ≤ -0.45, P < 0.05). Our results support previous estimates of interstitial glycerol concentration within adipose tissue and reveal capillary diffusion capacity is reduced in obesity.
- Subjects
LIPOLYSIS; ADIPOSE tissues; TRIGLYCERIDES; BLOOD flow; CAPILLARIES
- Publication
Diabetes, 2005, Vol 54, Issue 7, p1934
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/diabetes.54.7.1934