We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Gibberellin Signaling Promotes the Secondary Growth of Storage Roots in Panax ginseng.
- Authors
Hong, Chang Pyo; Kim, Jinsoo; Lee, Jinsu; Yoo, Seung-il; Bae, Wonsil; Geem, Kyoung Rok; Yu, Jin; Jang, Inbae; Jo, Ick Hyun; Cho, Hyunwoo; Shim, Donghwan; Ryu, Hojin
- Abstract
Gibberellins (GAs) are an important group of phytohormones associated with diverse growth and developmental processes, including cell elongation, seed germination, and secondary growth. Recent genomic and genetic analyses have advanced our knowledge of GA signaling pathways and related genes in model plant species. However, functional genomics analyses of GA signaling pathways in Panax ginseng, a perennial herb, have rarely been carried out, despite its well-known economical and medicinal importance. Here, we conducted functional characterization of GA receptors and investigated their physiological roles in the secondary growth of P. ginseng storage roots. We found that the physiological and genetic functions of P. ginseng gibberellin-insensitive dwarf1s (PgGID1s) have been evolutionarily conserved. Additionally, the essential domains and residues in the primary protein structure for interaction with active GAs and DELLA proteins are well-conserved. Overexpression of PgGID1s in Arabidopsis completely restored the GA deficient phenotype of the Arabidopsis gid1a gid1c (atgid1a/c) double mutant. Exogenous GA treatment greatly enhanced the secondary growth of tap roots; however, paclobutrazol (PCZ), a GA biosynthetic inhibitor, reduced root growth in P. ginseng. Transcriptome profiling of P. ginseng roots revealed that GA-induced root secondary growth is closely associated with cell wall biogenesis, the cell cycle, the jasmonic acid (JA) response, and nitrate assimilation, suggesting that a transcriptional network regulate root secondary growth in P. ginseng. These results provide novel insights into the mechanism controlling secondary root growth in P. ginseng.
- Subjects
GINSENG; FUNCTIONAL genomics; AMINO acid sequence; GENE regulatory networks; GERMINATION; ROOT growth; JASMONIC acid; SECONDARY metabolism
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 16, p8694
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22168694