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- Title
Transcriptome Analysis Implicates Involvement of Long Noncoding RNAs in Cytoplasmic Male Sterility and Fertility Restoration in Cotton.
- Authors
Zhang, Bingbing; Zhang, Xuexian; Zhang, Meng; Guo, Liping; Qi, Tingxiang; Wang, Hailin; Tang, Huini; Qiao, Xiuqin; Shahzad, Kashif; Xing, Chaozhu; Wu, Jianyong
- Abstract
The cytoplasmic male sterility (CMS)/restorer-of-fertility system is an important tool to exploit heterosis during commercially hybrid seed production. The importance of long noncoding RNAs (lncRNAs) in plant development is recognized, but few analyses of lncRNAs during anther development of three-line hybrid cotton (CMS-D2 line A, maintainer line B, restorer-of-fertility line R) have been reported. Here, we performed transcriptome sequencing during anther development in three-line hybrid cotton. A total of 80,695 lncRNAs were identified, in which 43,347 and 44,739 lncRNAs were differentially expressed in A–B and A–R comparisons, respectively. These lncRNAs represent functional candidates involved in CMS and fertility restoration. GO analysis indicated that cellular hormone metabolic processes and oxidation–reduction reaction processes might be involved in CMS, and cellular component morphogenesis and small molecular biosynthetic processes might participate in fertility restoration. Additionally, 63 lncRNAs were identified as putative precursors of 35 miRNAs, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed a similar expression pattern to RNA-seq data. Furthermore, construction of lncRNA regulatory networks indicated that several miRNA–lncRNA–mRNA networks might be involved in CMS and fertility restoration. Our findings provide systematic identification of lncRNAs during anther development and lays a solid foundation for the regulatory mechanisms and utilization in hybrid cotton breeding.
- Subjects
PLANT fertility; MALE sterility in plants; CYTOPLASMIC male sterility; NON-coding RNA; REVERSE transcriptase polymerase chain reaction; FERTILITY; OXIDATION-reduction reaction
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 22, p5530
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20225530