We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Impact of X-ray Exposure on the Proliferation and Differentiation of Human Pre-Adipocytes.
- Authors
Shreder, Kateryna; Rapp, Felicitas; Tsoukala, Ioanna; Rzeznik, Vanessa; Wabitsch, Martin; Fischer-Posovszky, Pamela; Fournier, Claudia
- Abstract
Radiotherapy is a widely used treatment option for cancer patients as well as for patients with musculoskeletal disorders. Adipocytes, the dominant cell type of adipose tissue, are known to constitute an active part of the tumor microenvironment. Moreover, adipocytes support inflammatory processes and cartilage degradation in chronic inflammatory diseases, i.e., rheumatoid and osteoarthritis. Since the production of inflammatory factors is linked to their differentiation stages, we set out to explore the radiation response of pre-adipocytes that may influence their inflammatory potential and differentiation capacity. This is the first study investigating the effects of X-ray irradiation on the proliferation and differentiation capacity of human primary pre-adipocytes, in comparison to Simpson–Golabi–Behmel Syndrome (SGBS) pre-adipocytes, an often-used in vitro model of human primary pre-adipocytes. Our results demonstrate a dose-dependent reduction of the proliferation capacity for both cell strains, whereas the potential for differentiation was mostly unaffected by irradiation. The expression of markers of adipogenic development, such as transcription factors (PPARγ, C/EBPα and C/EBPβ), as well as the release of adipokines (visfatin, adiponectin and leptin) were not significantly changed upon irradiation. However, after irradiation with high X-ray doses, an increased lipid accumulation was observed, which suggests a radiation-induced response of adipocytes related to inflammation. Our results indicate that pre-adipocytes are radio-resistant, and it remains to be elucidated whether this holds true for the overall inflammatory response of adipocytes upon irradiation.
- Subjects
CELL proliferation; CELL differentiation; MUSCULOSKELETAL system diseases; FAT cells; IRRADIATION
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 9, p2717
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19092717