We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of ACTH and hCG on the Expression of Gonadotropin-Inducible Ovarian Transcription Factor 1 (Giot1) Gene in the Rat Adrenal Gland.
- Authors
Jopek, Karol; Tyczewska, Marianna; Ramanjaneya, Manjunath; Szyszka, Marta; Celichowski, Piotr; Milecka, Paulina; Malendowicz, Ludwik K.; Rucinski, Marcin
- Abstract
Gonadotropin-inducible ovarian transcription factor-1 (<italic>Giot1</italic>) belongs to a family of fast-responsive genes, and gonadotropins rapidly induce its expression in steroidogenic cells of ovaries and testes of rats. Gonadal <italic>Giot1</italic> gene expression is regulated by cyclic adenosine monophosphate (cAMP) -dependent protein kinase A pathway, with essential role of orphan nuclear receptor NR4A1 transcription factor (nuclear receptor subfamily 4, group A, member 1). A recent study reports that <italic>Giot1</italic> is also expressed in adrenals, however, the mechanism of its regulation in adrenal gland is yet to be identified. Therefore, the aim of this study was to characterise the changes in <italic>Giot1</italic> gene expression in male and female rat adrenals using wide range of in vivo and in vitro experimental models. Special emphasis was directed at the <italic>Giot1</italic> gene regulation by ACTH and gonadotropin. In our study, we found that ACTH rapidly stimulates <italic>Giot1</italic> expression both in vivo and in vitro. However, gonadotropin does not affect the adrenal <italic>Giot1</italic> gene expression, presumably due to the low expression of gonadotropin receptor in adrenals. Both testosterone and estradiol administered in vivo had inhibitory effect on <italic>Giot1</italic> gene expression in the adrenals of post-gonadectomized adult rats. Further, our studies revealed that the intracellular mechanism of <italic>Giot1</italic> gene regulation in rat adrenals is similar to that of gonads. As in the case of gonads, the expression of <italic>Giot1</italic> in adrenal gland is regulated by cAMP-dependent signaling pathway with essential role of the NR4A1 transcription factor. The results of our studies suggest that <italic>Giot1</italic> may be involved in the regulation of rat adrenocortical steroidogenesis.
- Subjects
GONADOTROPIN; TRANSCRIPTION factors; CYCLIC adenylic acid; PROTEIN kinases; GENE expression; TESTOSTERONE
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 8, p2285
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19082285