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- Title
The Application of an Emerging Technique for Protein-Protein Interaction Interface Mapping: The Combination of Photo-Initiated Cross-Linking Protein Nanoprobes with Mass Spectrometry.
- Authors
Ptáèková, Renata; Jeèmen, Tomáš; Novák, Petr; Hudeèek, Jiøí; Stiborová, Marie; Šulc, Miroslav
- Abstract
Protein-protein interaction was investigated using a protein nanoprobe capable of photo-initiated cross-linking in combination with high-resolution and tandem mass spectrometry. This emerging experimental approach introduces photo-analogs of amino acids within a protein sequence during its recombinant expression, preserves native protein structure and is suitable for mapping the contact between two proteins. The contact surface regions involved in the well-characterized interaction between two molecules of human 14-3-3ζ regulatory protein were used as a model. The employed photo-initiated cross-linking techniques extend the number of residues shown to be within interaction distance in the contact surface of the 14-3-3ζ dimer (Gln8-Met78). The results of this study are in agreement with our previously published data from molecular dynamic calculations based on high-resolution chemical cross-linking data and Hydrogen/Deuterium exchange mass spectrometry. The observed contact is also in accord with the 14-3-3ζ X-ray crystal structure (PDB 3dhr). The results of the present work are relevant to the structural biology of transient interaction in the 14-3-3ζ protein, and demonstrate the ability of the chosen methodology (the combination of photo-initiated cross-linking protein nanoprobes and mass spectrometry analysis) to map the protein-protein interface or regions with a flexible structure.
- Subjects
MASS spectrometers; X-ray crystallography; PROTEIN-protein interactions; MOLECULAR dynamics; PROTEIN expression
- Publication
International Journal of Molecular Sciences, 2014, Vol 15, Issue 6, p9224
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms15069224