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- Title
Molecular Mechanisms of RADA16-1 Peptide on Fast Stop Bleeding in Rat Models.
- Authors
Ting Wang; Xiaozhong Zhong; Songtao Wang; Fei Lv; Xiaojun Zhao
- Abstract
Ionic self-assembly of the peptide RADARADARADARADA (RADA16-1) may form a well-defined nanofiber and eventually a hydrogel scaffold, with a water content of over 99.5%. This leads to the establishment of a nanofiber barrier that can be used to achieve complete hemostasis in less than 20 s in multiple tissues and in a variety of different wounds. In the present study, the nanofiber scaffolds of RADA16-1 peptide were sonicated into smaller fragments to identify possible molecular mechanisms underlying the rapid cessation of bleeding associated with these materials. Atomic force microscopy (AFM), circular dichroism (CD), and rheometry were also used to evaluate the re-assembly kinetics of this peptide. A bleeding control experiment was performed in animal models to uncover the molecular mechanisms underlying this fast hemostasis. In this way, these sonicated fragments not only quickly reassembled into nanofibers indistinguishable from the original material, but the degree of reassembly was also correlated with an increase in the rigidity of the scaffold and increased as the time required for hemostasis increased.
- Subjects
MOLECULAR biology; PEPTIDES; HEMORRHAGE; MOLECULAR self-assembly; NANOFIBERS; COLLOIDS; HEMOSTASIS; LABORATORY rats
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 11, p15279
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms131115279