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- Title
Aplicación de la técnica de inmunofluorescencia indirecta para la observación de levaduras en biopsias de pacientes con esporotricosis cutánea con el uso de anticuerpos antilevaduras y antiesporas.
- Authors
A., Palma-Ramos; L. E., Castrillón-Rivera; A., Paredes-Rojas; M. E., Vega-Memije; R., Arenas-Guzmán; J. I., Castañeda-Sánchez
- Abstract
BACKGROUND: Sporotrichosis is a fungal infection, acquired by traumatic inoculation or by inhalation of conidia of any of the species of Sporothrix schenckii complex; this complex includes: S. albicans, S. brasiliensis, S. globosa, S. luriei, S. mexicana and S. schenckii. This is a dimorphic fungus that occurs in nature in the mycelial form and when a host is infected, the fungus develops a yeast. Sporotrichosis has been classified in cutaneous and extracutaneous. Definitive laboratory diagnosis is performed by fungus culture, and sometimes by biopsy or PAS or Grocott smears; however, sometimes immunological procedures are mandatory. OBJECTIVE: To perform indirect immunofluorescence technique for the diagnosis of cutaneous sporotrichosis with the use of antibodies anti-yeast and anti-spore of Sporothrix schenckii complex. MATERIAL AND METHOD: An experimental and descriptive study in which ten biopsies from patients with diagnosis of cutaneous sporotrichosis from the Dermatology Department at Dr. Manuel Gea González General Hospital, Mexico City, were used. Three slices were made by biopsy, one for the PAS technique and two for indirect immunofluorescence for IgG anti-yeast and IgG anti-spore and then an IgG-FITC (fluorescein isotiocianate) made in goat anti-IgG rabbit. RESULTS: In all cases we found both the anti-yeast antibody and the anti-spore antibody recognized yeasts with excellent definition. CONCLUSION: The indirect immunofluorescence technique provides a method of high affinity, reliable and simple for the diagnosis of cutaneous sporotrichosis.
- Publication
Dermatología Revista Mexicana, 2016, Vol 60, Issue 5, p373
- ISSN
0185-4038
- Publication type
Article