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- Title
Study of stability of variants of ovine prions with different susceptibilities to scrapie.
- Authors
Rezaei, H.; Choiset, Y.; Debey, P.; Grosclaude, J.; Haertlé, T.
- Abstract
Well-defined set of sheep PrP polymorphisms at positions 136, 154 and 171 define susceptibility to scrapie, ranging from very high susceptibility observed for V136-R154-Q171 (VRQ) variant to resistance for A136-R154-R171 (ARR). To gain insight into the mechanisms of scrapie susceptibility/resistance, the unfolding pathways of the sheep prion protein variants were analysed by differential scanning calorimetry over a wide range of pH. Thermal unfolding occurs, in the 5.0 to 6.0 pH range, through a reversible one-step process while at pH<4.5 and >6.0 unfolding intermediates are formed, which are stable in the 65-80°C range. The observed differences correlate with ovine susceptibility to scrapie.
- Subjects
SCRAPIE; VIRUS diseases in sheep; DISEASE susceptibility; PRION diseases in animals; PRIONS; PROTEINS
- Publication
Journal of Thermal Analysis & Calorimetry, 2003, Vol 71, Issue 1, p237
- ISSN
1388-6150
- Publication type
Article
- DOI
10.1023/A:1022243006871