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- Title
A New Method for the Detection of Peptides and Similar Compounds on Paper Chromatograms.
- Authors
RYDON, H. N.; SMITH, P. W. G.
- Abstract
SINCE the first introduction1 of paper chromatography for the separation and identification of amino-acids and peptides, ninhydrin has been used almost exclusively as the spraying reagent to reveal the positions of the spots, although some use has been made of ultra-violet fluorescence2 and of spraying with potassium permanganate3 and specific reagents for individual amino-acids4. Ninhydrin, however, has two serious limitations: it does not react with cyclic peptides or with acylated amino-acids or peptides, while with peptides, since only the terminal amino-group enters into the reaction, the colour developed with a given amount of material decreases with increasing molecular weight.
- Publication
Nature, 1952, Vol 169, Issue 4309, p922
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/169922b0