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- Title
基于代谢组学技术的金刚烷胺胁迫刺参的毒性作用机 制研究.
- Authors
赵军强; 韩典峰; 田秀慧; 刘鸽; 刘小静; 高永刚; 姜芳; 刘欢; 崔艳梅; 罗晶晶; 洪赫阳; 徐英江
- Abstract
To explore the toxicity of amantadine on intestinal tissue of Apostichopus japonicus and the related mo-lecular mechanisms, the composition and content of metabolites were analyzed based on non-targeted metabolomics methods after the sea cucumbers were exposed to 100 μg·L-1 amantadine for 96 h. Metabolomics results showed that intestinal metabolites of A. japonicus were changed under amantadine stress, and there were 115 differentially expressed metabolites compared with the control group, whose main functions were dipeptide, steroid, purine and pyrimidine. The KEGG enrichment analysis showed that biological processes were significantly affected, such as amino acid metabolism, lipid metabolism, signal transduction, nucleotide metabolism and the endocrine system. According to the physiological functions of the selected differential metabolites and the metabolic pathways involved, it was found that the signal transduction and energy metabolism were disturbed, possibly due to the decrease of adenosine and adenosine monophosphate (AMP) content. The decrease in testosterone and taurochenodeoxycholic acid (TCDCA) content affected sex differentiation and cholesterol metabolism of A. japonicus. The increase in gly-cine and proline content might be caused by the degradation of the collagen of A. japonicus. This research explored the molecular mechanism of amantadine on intestinal tissue and the response regulation mechanism of A. japonicus, and provided the theoretical basis for further research on the toxic mechanism of amantadine.
- Subjects
AMINO acid metabolism; APOSTICHOPUS japonicus; CHOLESTEROL metabolism; ADENOSINE monophosphate; SEA cucumbers; ENERGY metabolism
- Publication
Asian Journals of Ecotoxicology, 2023, Vol 18, Issue 2, p15
- ISSN
1673-5897
- Publication type
Article
- DOI
10.7524/AJE.1673-5897.20220718001