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- Title
Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay.
- Authors
Hiroaki Tachiwana; Dacher, Mariko; Kazumitsu Maehara; Akihito Harada; Yosuke Seto; Ryohei Katayama; Yasuyuki Ohkawa; Hiroshi Kimura; Hitoshi Kurumizaka; Noriko Saitoh
- Abstract
In eukaryotes, histone variant distribution within the genome is the key epigenetic feature. To understand how each histone variant is targeted to the genome, we developed a new method, the RhIP (Reconstituted histone complex Incorporation into chromatin of Permeabilized cell) assay, in which epitope-tagged histone complexes are introduced into permeabilized cells and incorporated into their chromatin. Using this method, we found that H3.1 and H3.3 were incorporated into chromatin in replication-dependent and -independent manners, respectively. We further found that the incorporation of histones H2A and H2A.Z mainly occurred at less condensed chromatin (open), suggesting that condensed chromatin (closed) is a barrier for histone incorporation. To overcome this barrier, H2A, but not H2A.Z, uses a replication-coupled deposition mechanism. Our study revealed that the combination of chromatin structure and DNA replication dictates the differential histone deposition to maintain the epigenetic chromatin states.
- Subjects
HISTONES; CHROMATIN; DNA replication; DNA structure
- Publication
eLife, 2021, p1
- ISSN
2050-084X
- Publication type
Article
- DOI
10.7554/eLife.66290