We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Responses of fish chromatophore-based cytosensor to a broad range of biological agents.
- Authors
Karen P. Dierksen; Ljiljana Mojovic; Bruce A. Caldwell; R. Ryan Preston; Rosalyn Upson; Jeannine Lawrence; Philip N. McFadden; Janine E. Trempy
- Abstract
A cytosensor based on living chromatophores from Betta splendens Siamese ?ghting ?sh was used to test several classes of biologically active agents. Tested agents include neurotransmitters, adenyl cyclase activators, cytoskeleton effectors, cell membrane effectors and protein synthesis inhibitors. Characteristic cell responses were analyzed, and potential cytosensor applications were considered. Streptococcus pyogenes toxins streptolysin S and streptolysin O, Clostridium tetani tetanolysin, Staphylococcus aureus alpha-toxin and Vibrio parahemolyticus hemolysin, all bacterial toxins that act on cell membranes, elicited a strong response from chromatophores. A comparison of puri?ed toxin to actual bacterial culture from Vibrio parahemolyticus demonstrated a nearly identical chromatophore cell response pattern. This suggests that the cytosensor response is re?ective of bacterial toxin production. Copyright 2004 John Wiley & Sons, Ltd.
- Subjects
CHROMATOPHORES; EPITHELIAL cells; PLASTIDS; PROTOPLASM
- Publication
Journal of Applied Toxicology, 2004, Vol 24, Issue 5, p363
- ISSN
0260-437X
- Publication type
Article
- DOI
10.1002/jat.1030