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- Title
Single colony genetic analysis of epilithic stream algae of the genus <italic>Chamaesiphon</italic> spp.
- Authors
Kurmayer, Rainer; Christiansen, Guntram; Holzinger, Andreas; Rott, Eugen
- Abstract
In order to understand <italic>Chamaesiphon</italic> spp. evolution and ecological diversification, we investigated the phylogenetic differentiation of three morphospecies from field samples by means of single colony genetics. Individual colonies of three different morphospecies (<italic>C. starmachii, C. polonicus, C. geitleri,</italic>) were isolated from lotic gravel streams and their 16S rDNA nucleotide variability was analyzed. For a number of individual colonies, microscopical and ultrastructural analysis was also performed. A phylogenetic tree of all major lineages of the phylum of Cyanobacteria assigned all <italic>Chamaesiphon</italic> genotypes (1149-1176 bp) most closely with the family of Gomontiellaceae of the order Oscillatoriales. The sequences obtained from colonies assigned to <italic>C. starmachii</italic> (<italic>n</italic> = 21), <italic>C. polonicus</italic> (<italic>n</italic> = 9), and <italic>C. geitleri</italic> (<italic>n</italic> = 17) were found to reveal high average (3.5%) nucleotide diversity. No phylogenetic sub-branching in correspondence with morphology was observed suggesting that the three <italic>Chamaesiphon</italic> morphospecies did not represent monophyletic taxa. We could not attribute specific thylakoid ultrastructure to phylogenetic sub-branches; however, the observed parietally and loosely arranged thylakoids indicate that for the genus <italic>Chamaesiphon</italic>, the variability in thylakoid ultrastructure might have been underestimated. In summary, the high nucleotide diversity of the 16S rDNA gene implies phylogenetic diversity that corresponds little to morphological classification.
- Subjects
PHYLOGENY; COLONIES (Biology); BACTERIAL colonies; NUCLEOTIDES; CELL morphology
- Publication
Hydrobiologia, 2018, Vol 811, Issue 1, p61
- ISSN
0018-8158
- Publication type
Article
- DOI
10.1007/s10750-017-3295-z