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- Title
Engineering Enzyme Stability and Resistance to an Organic Cosolvent by Modification of Residues in the Access Tunnel.
- Authors
Koudelakova, Tana; Chaloupkova, Radka; Brezovsky, Jan; Prokop, Zbynek; Sebestova, Eva; Hesseler, Martin; Khabiri, Morteza; Plevaka, Maryia; Kulik, Daryna; Kuta Smatanova, Ivana; Rezacova, Pavlina; Ettrich, Rudiger; Bornscheuer, Uwe T.; Damborsky, Jiri
- Abstract
Mutations targeting as few as four residues lining the access tunnel extended the half‐life of an enzyme in 40 % dimethyl sulfoxide from minutes to weeks and increased its melting temperature by 19 °C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active‐site accessibility for cosolvent molecules (red dots).
- Publication
Angewandte Chemie International Edition, 2013, Vol 52, Issue 7, p1959
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201206708