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- Title
Circadian variations in biochemical markers of bone metabolism in horse of different age.
- Authors
Giannetto, Claudia; Casella, Stefania; Fazio, Francesco; Messina, Vanessa; Piccione, Giuseppe
- Abstract
The author studied in thirty Thoroughbred horses the influence of age and gender on daily rhythms of serum osteocalcin (OC). The animals were divided into two groups. Group A: six male and six female 2 years old; Group B: six male, six female and six geldings 6 years old. They were housed individually in box-like stalls under natural photoperiod and environmental conditions. Blood samples were collected every 3 hours over a 48 hour period. Statistical differences of serum OC concentration due to different gender were observed in both Groups A and B. Daily rhythms of serum OC concentration were observed only in Group B, with nocturnal acrophase. In females the acrophase was statistically postponed for 1 hour compared to male and gelding. Male and female showed a more robust daily rhythm than the geldings. The results showed that blood sampling for determination of serum OC should be strictly standardized with regard to the time of the day.
- Subjects
THOROUGHBRED horse; BIOMARKERS; BIOLOGICAL rhythms; AGE; GENDER; BLOOD testing
- Publication
Journal of Applied Biomedicine, 2010, Vol 8, Issue 2, p73
- ISSN
1214-021X
- Publication type
Article
- DOI
10.2478/v10136-009-0011-y