We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Physiogenomic resources for rat models of heart, lung and blood disorders.
- Authors
Malek, Renae L.; Hong-ying Wang; Kwitek, Anne E.; Greene, Andrew S.; Bhagabati, Nirmal; Borchardt, Gretta; Cahill, Lisa; Currier, Tracey; Frank, Bryan; Xianping Fu; Hasinoff, Michael; Howe, Eleanor; Letwin, Noah; Luu, Truong V.; Saeed, Alexander; Sajadi, Hedieh; Salzberg, Steven L.; Sultana, Razvan; Thiagarajan, Mathangi; Tsai, Jennifer
- Abstract
Cardiovascular disorders are influenced by genetic and environmental factors. The TIGR rodent expression web-based resource (TREX) contains over 2,200 microarray hybridizations, involving over 800 animals from 18 different rat strains. These strains comprise genetically diverse parental animals and a panel of chromosomal substitution strains derived by introgressing individual chromosomes from normotensive Brown Norway (BN/NHsdMcwi) rats into the background of Dahl salt sensitive (SS/JrHsdMcwi) rats. The profiles document gene-expression changes in both genders, four tissues (heart, lung, liver, kidney) and two environmental conditions (normoxia, hypoxia). This translates into almost 400 high-quality direct comparisons (not including replicates) and over 100,000 pairwise comparisons. As each individual chromosomal substitution strain represents on average less than a 5% change from the parental genome, consomic strains provide a useful mechanism to dissect complex traits and identify causative genes. We performed a variety of data-mining manipulations on the profiles and used complementary physiological data from the PhysGen resource to demonstrate how TREX can be used by the cardiovascular community for hypothesis generation.
- Subjects
HEART abnormalities; GENETICS; BIOLOGY; SPECIES hybridization; BREEDING; HEREDITY; CHROMOSOMES; ANIMAL research; RATS; RODENTS
- Publication
Nature Genetics, 2006, Vol 38, Issue 2, p234
- ISSN
1061-4036
- Publication type
Article
- DOI
10.1038/ng1693