We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Frequency of Hepatitis C Virus Genotypes /Subtypes Association with Response to Therapy in a Sample of HCV Infected Iraqi Patients.
- Authors
Abdulhassan, Laith J.; Abdulamir, Ahmed S.; Alkhalidi, Nawal M.; Alwaysi, Safaa A.
- Abstract
Background Hepatitis C virus (HCV) is an important human pathogen affecting 120-170 million individuals in the world. Identification of the causative virus genotype is of a significance to both clinical practices and predict the likelihood to therapy response. Objective To determine the distribution of HCV genotypes/subtypes and its association with response to therapy among newly diagnosed HCV patients. Methods Fifty patients with confirmed anti-HCV antibodies were included in this study for HCV genotyping in association with response to therapy. Blood samples from patients were subjected to RNA extraction and reverse transcription step; viral load of HCV was measured by polymerase chain reaction (PCR) at time zero, 3 months and 6 months of dual therapy. Response to therapy was measured as a decrease in viral load (2 log or more) and was described as: good (median log is zero after 6 months of therapy), moderate (median log declines more than 2 log but not zero after 6 months of therapy), poor (median log does not decline or decline less than 2 log after 6 months of therapy). Results Two genotypes of HCV were detected, genotype 4 was the predominant (27/50, 54%) followed by genotype 1 (23/50, 46%). For HCV subtypes, subtype 1a was of highest percentage (28%) followed by 4e (24%), 1b (18%), 4a (14%), 4b (12%), and 4e (4%).The results revealed a significant association between HCV subtypes, but not genotypes, with response to therapy. HCV subtype 1a followed by 4a showed the highest rate of response 85.7% and 71.4%, respectively, while interestingly HCV subtype 4d showed no response and 1b showed poor response 11.11%. Conclusion HCV subtypes of great importance in predicting success to HCV therapy and it is believed this would affect the newly emerging directly acting drugs as well.
- Subjects
HEPATITIS C virus; GENOTYPES; VIRAL hepatitis; REVERSE transcriptase polymerase chain reaction; THERAPEUTICS
- Publication
Iraqi Journal of Medical Sciences, 2018, Vol 16, Issue 1, p30
- ISSN
1681-6579
- Publication type
Article
- DOI
10.22578/IJMS.16.1.6