We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The B-cell compartment in antibody-deficient infants and young children -- developing common variable immunodeficiency or transient immune maturation?
- Authors
Szczawinska-Poplonyk, Aleksandra; Tapolska-Jozwiak, Katarzyna; Samara, Husam
- Abstract
Background: Hypogammaglobulinemia in early childhood is a common feature characterized by distinct intrinsic and extrinsic factors leading to disturbed peripheral blood lymphocyte homeostasis. Detailed flow cytometric immunophenotyping of the peripheral blood B cell compartment is an informative tool for delineating disturbed generation of B cell subpopulations crucial for the diagnosis of hypogammaglobulinemia in young children. Methods: We analyzed by flow cytometry the proportions and absolute values of total, naïve, memory - non-switched and switched, transitional and immature B lymph cells as well as plasmablasts in the peripheral blood of 50 hypogammaglobulinemic children aged from 3 to 50 months. Results: Beyond physiological, age-related changes within the B cell pool, a proportion of children manifested defective differentiation into switched memory and accumulation of CD21lo immature B cells. Conclusions: Dynamic shifts within B cell subpopulations of the immature immune system being most prominent during the first two years of life contribute to the age-related developmental abnormalities of the B cell compartment. Therefore, a reliable diagnosis of common variable immunodeficiency (CVID) in young hypogammaglobulinemic children cannot yet be established despite their clinical and immunological phenotypes sharing common features with this primary immunodeficiency.
- Subjects
ANTIGEN analysis; AGAMMAGLOBULINEMIA; ACADEMIC medical centers; B cells; CELL differentiation; CHI-squared test; DIFFERENTIAL diagnosis; FLOW cytometry; IMMUNE system; IMMUNOPHENOTYPING; COMMON variable immunodeficiency; PROBABILITY theory; RESEARCH funding; RETROSPECTIVE studies; DATA analysis software; DESCRIPTIVE statistics; CHILDREN; DIAGNOSIS
- Publication
Italian Journal of Pediatrics, 2016, Vol 42, p1
- ISSN
1720-8424
- Publication type
Article
- DOI
10.1186/s13052-016-0279-y