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- Title
Inducers and autoinducers on Salmonella enterica serovar Typhimurium motility, growth and gene expression.
- Authors
dos Santos da Conceição, Rita de Cássia; Tuchtenhagen Sturbelle, Régis; Dias Timm, Cláudio; Leivas Leite, Fábio Pereira
- Abstract
Genus Salmonella bacteria are among the major pathogenic microorganisms in food. This bacterium pathogenicity is related to a number of virulence factors, among which itsflagella. Flagellum expression is one of the virulence factors modulated by Quorum Sensing. Epinephrine produced by mammals uses the same signaling pathway of the 3 bacteria autoinducer. This study evaluated the effect of molecules inducer (epinephrine) and autoinducers (autoinducer 2 and autoinducer 3) and their association with the motility, growth and expression genes JihC, fliA, fliY, motA, motB e fliC of Salmonella Typhimurium (ST). Initially, ST was inoculated in BHI. Then, motility assays, growth curves and gene expression were performed by testing different concentrations of epinephrine (50, 125, 250, 500µM), conditioned medium (10 and 50%) and a combination of these. STwas exposed to different concentrations of epinephrine, conditioned medium and an association of both. Following, motility assays, bacterial growth and gene expression were performed. The results obtained showed that the combination of 500µM epinephrine with 50% conditioned medium increased ST bacterial motility by increasing the expression of genes involved in flagellum assembly.
- Subjects
SALMONELLA enterica; MOTILITY of bacteria; BACTERIAL growth; GENE expression in bacteria; FOOD microbiology
- Publication
Ciência Rural, 2015, Vol 45, Issue 12, p2201
- ISSN
0103-8478
- Publication type
Article
- DOI
10.1590/0103-8478cr20150295