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- Title
Improved clinical outcomes of preimplantation genetic testing for aneuploidy using MALBAC-NGS compared with MDA-SNP array.
- Authors
Niu, Wenbin; Wang, Linlin; Xu, Jiawei; Li, Ying; Shi, Hao; Li, Gang; Jin, Haixia; Song, Wenyan; Wang, Fang; Sun, Yingpu
- Abstract
<bold>Background: </bold>To assess whether preimplantation genetic testing for aneuploidy with next generation sequencing (NGS) outweighs single nucleotide polymorphism (SNP) array in improving clinical outcomes.<bold>Methods: </bold>A retrospective analysis of the clinical outcomes of patients who underwent PGT-A treatment in a single center from January 2013 to December 2017.A total of 1418 couples who underwent PGT-A treatment were enrolled, of which 805 couples used NGS for PGT-A, while the remaining 613 couples used SNP array for PGT-A. Clinical pregnancy rate, miscarriage rate and healthy baby rate were compared between the MALBAC-NGS-PGT-A and MDA-SNP-PGT-A groups.<bold>Results: </bold>After testing karyotypes of 5771 biopsied blastocysts, 32.2% (1861/5771) were identified as chromosomally normal, while 67.8% were chromosomally abnormal. In terms of clinical outcomes, women in the MALBAC-NGS-PGT-A group had a significantly higher clinical pregnancy rate (50.5% vs 41.7%, p = 0.002) and healthy baby rate (39.6% vs 31.4%, p = 0.003), and a lower miscarriage rate (15.5% vs 22.8%, p = 0.036).<bold>Conclusion: </bold>This is the largest study reporting the extensive application of NGS-based PGT-A, whilst comparing the clinical outcomes of MALBAC-NGS-PGT-A and MDA-SNP-PGT-A. The results provide greater evidence supporting the wider use of NGS in PGT-A, not only for its lower cost but also for its improved clinical outcomes compared to SNP-based PGT-A.
- Subjects
CLINICAL trials; GENETIC testing; SINGLE nucleotide polymorphisms; NUCLEOTIDE sequencing; BIOPSY
- Publication
BMC Pregnancy & Childbirth, 2020, Vol 20, Issue 1, p1
- ISSN
1471-2393
- Publication type
journal article
- DOI
10.1186/s12884-020-03082-9