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- Title
Pulsatile ex vivo perfusion of human saphenous vein grafts under controlled pressure conditions increases MMP-2 expression.
- Authors
Dummler, Sara; Eichhorn, Stefan; Tesche, Christian; Schreiber, Ulrich; Voss, Bernhard; Deutsch, Marcus-André; Hauner, Hans; Lahm, Harald; Lange, Rüdiger; Krane, Markus; Deutsch, Marcus-André; Lange, Rüdiger
- Abstract
<bold>Background: </bold>The use of human saphenous vein grafts (HSVGs) as a bypass conduit is a standard procedure in the treatment of coronary artery disease while their early occlusion remains a major problem.<bold>Methods: </bold>We have developed an ex vivo perfusion system, which uses standardized and strictly controlled hemodynamic parameters for the pulsatile and non-static perfusion of HSVGs to guarantee a reliable analysis of molecular parameters under different pressure conditions. Cell viability of HSVGs (n = 12) was determined by the metabolic conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) into a purple formazan dye.<bold>Results: </bold>Under physiological flow rates (10 mmHg) HSVGs remained viable for two weeks. Their exposure to arterial conditions (100 mmHg) was possible for one week without important reduction in viability. Baseline expression of matrix metalloproteinase-2 (MMP-2) after venous perfusion (2.2 ± 0.5, n = 5) was strongly up-regulated after exposure to arterial conditions for three days (19.8 ± 4.3) or five days (23.9 ± 6.1, p < 0.05). Zymographic analyses confirmed this increase on the protein level. Our results suggest that expression and activity of MMP-2 are strongly increased after exposure of HSVGs to arterial hemodynamic conditions compared to physiological conditions.<bold>Conclusion: </bold>Therefore, our system might be helpful to more precisely understand the molecular mechanisms leading to an early failure of HSVGs.
- Subjects
SAPHENOUS vein; LEG blood-vessels; CORONARY disease; HEMODYNAMICS; METALLOPROTEINASES; BIOCHEMISTRY; IN vitro studies; RESEARCH; PHYSICS; ARTERIES; RESEARCH methodology; PROTEOLYTIC enzymes; PRESSURE; MEDICAL cooperation; EVALUATION research; TREATMENT failure; PHENOMENOLOGY; COMPARATIVE studies; CORONARY artery disease; TRANSPLANTATION of organs, tissues, etc.
- Publication
BioMedical Engineering OnLine, 2011, Vol 10, Issue 1, p62
- ISSN
1475-925X
- Publication type
journal article
- DOI
10.1186/1475-925X-10-62