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- Title
Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particles.
- Authors
Kulpa, Deanna A.; Moran, John V.
- Abstract
LINE-1 retrotransposons (L1s) constitute ∼17% of human DNA, and their activity continues to affect genome evolution. Retrotransposition-competent human L1s encode two proteins required for their mobility (ORF1p and ORF2p); however, biochemical activities associated with ORF2p have been difficult to detect in cells. Here, we show for the first time the colocalization of L1 RNA, ORF1p and ORF2p to a putative ribonucleoprotein retrotransposition intermediate. We further demonstrate that ORF2p preferentially uses its encoding RNA as a template for reverse transcription. Thus, our data provide the first biochemical evidence supporting the cis-preferential action of the L1 reverse transcriptase.
- Subjects
RIBOSOMES; DNA; GENOMES; PROTEIN synthesis; BIOCHEMICAL genetics; NUCLEOPROTEINS
- Publication
Nature Structural & Molecular Biology, 2006, Vol 13, Issue 7, p655
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb1107