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- Title
Suppression of Liquid‐Liquid Phase Separation and Aggregation of Antibodies by Modest Pressure Application.
- Authors
Fetahaj, Zamira; Jaworek, Michel W.; Oliva, Rosario; Winter, Roland
- Abstract
The high colloidal stability of antibody (immunoglobulin) solutions is important for pharmaceutical applications. Inert cosolutes, excipients, are generally used in therapeutic protein formulations to minimize physical instabilities, such as liquid–liquid phase separation (LLPS), aggregation and precipitation, which are often encountered during manufacturing and storage. Despite their widespread use, a detailed understanding of how excipients modulate the specific protein‐protein interactions responsible for these instabilities is still lacking. In this work, we demonstrate the high sensitivity to pressure of globulin condensates as a suitable means to suppress LLPS and subsequent aggregation of concentrated antibody solutions. The addition of excipients has only a minor effect. The high pressure sensitivity observed is due to the fact that these flexible Y‐shaped molecules create a considerable amount of void volume in the condensed phase, leading to an overall decrease in the volume of the system upon dissociation of the droplet phase by pressure already at a few tens of to hundred bar. Moreover, we show that immunoglobulin molecules themselves are highly resistant to unfolding under pressure, and can even sustain pressures up to about 6 kbar without conformational changes. This implies that immunoglobulins are resistant to the pressure treatment of foods, such as milk, in high‐pressure food‐processing technologies, thereby preserving their immunological activity.
- Subjects
PHASE separation; CONDENSED matter; COLLOIDAL stability; PROTEIN-protein interactions; GLOBULINS; IMMUNOGLOBULINS
- Publication
Chemistry - A European Journal, 2022, Vol 28, Issue 48, p1
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.202201658