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- Title
Evaluation of aroma active compounds in Tuber fruiting bodies by gas chromatography-olfactometry in combination with aroma reconstitution and omission test.
- Authors
Liu, Rui-Sang; Li, Dao-Cheng; Li, Hong-Mei; Tang, Ya-Jie
- Abstract
The aroma active compounds of three Tuber fruiting bodies (i.e., Tuber himalayense, Tuber indicum, and Tuber sinense) were firstly systematically evaluated by instrumental gas chromatography-olfactometry combining with quantitative analysis, aroma reconstitution, and omission tests. Twelve aroma active compounds were characterized by aroma extract dilution analysis, and 3-(methylthio) propanal, 3-methylbutanal, and 1-octen-3-ol with the highest flavor dilution (FD) factor (i.e., 1,024-2,048) were suggested as key contributors to the aroma. Odor activity value (OAV) was employed to determine the relative contribution of each compound to the aroma, and the compound with the highest FD factor also had the highest OAV (i.e., 10,234-242,951). Then, the synthetic blends of odorants (aroma reconstitution) were prepared with OAV larger than 15, and their aromas were very similar to the originals. Omission tests were carried out to verify the significance of 3-(methylthio) propanal, 1-octen-3-ol, and 3-methylbutanal as key compounds in the aroma of tested Tuber fruiting bodies.
- Subjects
AROMATIC compounds; GAS chromatography; OLFACTOMETRY; TUBERS; QUANTITATIVE research
- Publication
Applied Microbiology & Biotechnology, 2012, Vol 94, Issue 2, p353
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-011-3837-7