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- Title
Effect of CO[sub 2] Pneumoperitoneum on the Systemic and Peritoneal Cytokine Response in a LPS-Induced Sepsis Model.
- Authors
Matsumoto, Toshifumi; Dolgor, B.; Ninomiya, K.; Bandoh, T.; Yoshida, T.; Kitano, S.
- Abstract
We studied the effect of carbon dioxide (CO[sub 2] ) pneumoperitoneum on the systemic and peritoneal cytokine response in a rat model of intraperitoneal sepsis. After intraperitoneal injection of bacterial lipopolysaccharide (LPS, 10 mg/kg), rats were divided into 3 groups (n = 49 in each group): control (abdominal puncture); CO[sub 2] pneumoperitoneum, and laparotomy. Blood and peritoneal lavage fluid (PLF) were sampled at 0, 1, 2, 3, 4, 6, and 8 h after LPS challenge. Blood cell counts, plasma endotoxin level, and the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the plasma and PLF were measured. Blood cell counts did not differ between the 3 groups. Plasma endotoxin levels in the pneumoperitoneum group were significantly increased immediately after the procedure (p < 0.05). Although peak plasma TNF-α levels in the pneumoperitoneum group were seen immediately after the procedure, other changes in plasma cytokine levels did not differ significantly between the 3 groups. PLF TNF-α and IL-1β levels in the pneumoperitoneum group were significantly lower than levels in the control and laparotomy groups soon after the procedure (p < 0.05). PLF IL-6 levels in the pneumoperitoneum group tended to be lower than those in the laparotomy group. In conclusion, CO[sub 2] pneumoperitoneum might induce different responses between systemic and peritoneal cytokines soon after the procedure in a rat model of intraperitoneal sepsis.Copyright © 2001 S. Karger AG, Basel
- Subjects
ARTIFICIAL pneumoperitoneum; PHYSIOLOGICAL effects of carbon dioxide; ABDOMINAL surgery; CYTOKINES; TUMOR necrosis factors; INTERLEUKIN-1; INTERLEUKIN-6
- Publication
European Surgical Research, 2001, Vol 33, Issue 2, p71
- ISSN
0014-312X
- Publication type
Article
- DOI
10.1159/000049697