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- Title
A gene chimaera of SV40 and mouse β-globin is transcribed and properly spliced.
- Authors
Chu, Gilbert; Sharp, Phillip A.
- Abstract
A gene chimaera of the first exon from simian virus 40 (SV40) sequences coding for T antigen placed upstream from the third exon from mouse β-globin, and separated by the resultant new chimaeric intron, was cloned into a bacterial plasmid. When transfected into monkey cells, the gene chimaera was transcribed, polyadenylated and spliced using the donor splice site from SV40 and the acceptor splice site from mouse β-globin. This result suggests that a donor site from one gene can be spliced to an acceptor site from another gene.
- Publication
Nature, 1981, Vol 289, Issue 5796, p378
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/289378a0