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- Title
Glycosylation-related gene expression profiling in the brain and spleen of scrapie-affected mouse.
- Authors
Florence Guillerme-Bosselut; Lionel Forestier; Chantal Jayat-Vignoles; Jean-Luc Vilotte; Iuliana Popa; Jacques Portoukalian; Annick Le Dur; Hubert Laude; Raymond Julien; Paul-François Gallet
- Abstract
A central event in the formation of infectious prions is the conformational change of a host-encoded glycoprotein, PrPC, into a pathogenic isoform, PrPSc. The molecular requirements for efficient PrP conversion remain unknown. Altered glycosylation has been linked to various pathologies and the N-glycans harbored by two prion protein isoforms are different. In order to search for glycosylation-related genes that could mark prion infection, we used a glycosylation-dedicated microarray that allowed the simultaneous analysis of the expression of 165 glycosylation-related genes encoding proteins of the glycosyltransferase, glycosidase, lectin, and sulfotransferase families to compare the gene expression profiles of normal and scrapie-infected mouse brain and spleen. Eight genes were found upregulated in “scrapie brain” at the final state of the disease. In the spleen, five genes presented a modified expression. Three genes were also upregulated in the spleen of infected mice, and two (Pigq and St3gal5) downregulated. All changes were confirmed by qPCR and biochemical analyses applied to Pigq and St3gal5 proteins.
- Subjects
GLYCOSYLATION; GENETIC regulation; BRAIN physiology; SPLEEN physiology; SCRAPIE; LABORATORY mice; PRIONS; PROTEIN conformation; GLYCOPROTEINS; GENETICS
- Publication
Glycobiology, 2009, Vol 19, Issue 8, p879
- ISSN
0959-6658
- Publication type
Article
- DOI
10.1093/glycob/cwp062