We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes.
- Authors
Olexiková, Lucia; Dujíčková, Linda; Makarevich, Alexander V.; Bezdíček, Jiří; Sekaninová, Jana; Nesvadbová, Andrea; Chrenek, Peter
- Abstract
Vitrification of bovine oocytes can impair subsequent embryo development mostly due to elevated oxidative stress. This study was aimed at examining whether glutathione, a known antioxidant, can improve further embryo development when added to devitrified oocytes for a short recovery period. Bovine in vitro matured oocytes were vitrified using an ultra-rapid cooling technique on electron microscopy grids. Following warming, the oocytes were incubated in the recovery medium containing glutathione (0, 1.5, or 5 mmol L−1) for 3 h (post-warm recovery). Afterwards, the oocytes were lysed for measuring the total antioxidant capacity (TAC), activity of peroxidase, catalase and glutathione reductase, and ROS formation. The impact of vitrification on mitochondrial and lysosomal activities was also examined. Since glutathione, added at 5 mmol L−1, significantly increased the TAC of warmed oocytes, in the next set of experiments this dose was applied for post–warm recovery of oocytes used for IVF. Glutathione in the recovery culture did not change the total blastocyst rate, while increased the proportion of faster developing blastocysts (Day 6–7), reduced the apoptotic cell ratio and reversed the harmful impact of vitrification on the actin cytoskeleton. These results suggest that even a short recovery culture with antioxidant(s) can improve the development of bovine devitrified oocytes.
- Subjects
GLUTATHIONE; VITRIFICATION; OXIDANT status; ELECTRON microscope techniques; GLUTATHIONE reductase; OVUM; LYSOSOMES
- Publication
Antioxidants, 2023, Vol 12, Issue 1, p35
- ISSN
2076-3921
- Publication type
Article
- DOI
10.3390/antiox12010035