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- Title
Müller cell gliosis in retinal organ culture mimics gliotic alterations after ischemia in vivo
- Authors
Kuhrt, Heidrun; Wurm, Antje; Karl, Anett; Iandiev, Ianors; Wiedemann, Peter; Reichenbach, Andreas; Bringmann, Andreas; Pannicke, Thomas
- Abstract
Abstract: A decrease in the expression of inwardly rectifying potassium (Kir) currents is a characteristic feature of retinal glial (Müller) cells in various retinopathies, e.g., after transient retinal ischemia. We used short-term retinal organ cultures to investigate whether similar physiological alterations can be induced under in vitro conditions. During 4 days in vitro, Müller cells displayed a decrease in Kir currents and an increase in transient A-type potassium currents which was similar to the alterations in membrane physiology during ischemia-reperfusion in vivo. In addition, gliosis of Müller cells both in vivo and in organ cultures was associated with cellular hypertrophy and an alteration in osmotic swelling characteristics. Whereas Müller cells in control retinae did not swell under hypotonic stress, cells in postischemic retinae and in organ cultures swelled upon hypotonic challenge. Therefore, Müller cells in organ cultures can be used to investigate distinct aspects of ischemia-induced Müller cell gliosis. Both the decrease in Kir currents and the alteration in osmotic swelling may reflect a dysfunction of Müller cells regarding the control of the ionic and osmotic homeostasis in the retina.
- Subjects
NEUROSCIENCES; MEDICAL sciences; CELLS; ISCHEMIA
- Publication
International Journal of Developmental Neuroscience, 2008, Vol 26, Issue 7, p745
- ISSN
0736-5748
- Publication type
Article
- DOI
10.1016/j.ijdevneu.2008.07.003