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- Title
Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane.
- Authors
Huang, Yu-Chih; Huang, Kuen-Yu; Yang, Bing-Yuan; Ko, Chun-Han; Huang, Haw-Ming
- Abstract
An antiadhesion barrier membrane is an important biomaterial for protecting tissue from postsurgical complications. However, there is room to improve these membranes. Recently, carboxymethylcellulose (CMC) incorporated with hyaluronic acid (HA) as an antiadhesion barrier membrane and drug delivery system has been reported to provide excellent tissue regeneration and biocompatibility. The aim of this study was to fabricate a novel hydrogel membrane composed of berberine-enriched CMC prepared from bark of the P. amurense tree and HA (PE-CMC/HA). In vitro anti-inflammatory properties were evaluated to determine possible clinical applications. The PE-CMC/HA membranes were fabricated by mixing PE-CMC and HA as a base with the addition of polyvinyl alcohol to form a film. Tensile strength and ultramorphology of the membrane were evaluated using a universal testing machine and scanning electron microscope, respectively. Berberine content of the membrane was confirmed using a UV-Vis spectrophotometer at a wavelength of 260 nm. Anti-inflammatory property of the membrane was measured using a Griess reaction assay. Our results showed that fabricated PE-CMC/HA releases berberine at a concentration of 660 μg/ml while optimal plasticity was obtained at a 30 : 70 PE-CMC/HA ratio. The berberine-enriched PE-CMC/HA had an inhibited 60% of inflammation stimulated by LPS. These results suggest that the PE-CMC/HA membrane fabricated in this study is a useful anti-inflammatory berberine release system.
- Subjects
TISSUE adhesions; POSTOPERATIVE care; ALKALOIDS; ANIMAL experimentation; ANTI-inflammatory agents; BARK; BIOLOGICAL assay; BIOMEDICAL materials; CELL culture; CELL physiology; CELLULOSE; DRUG delivery systems; PHARMACEUTICAL gels; HYALURONIC acid; KINEMATICS; MATERIALS testing; ARTIFICIAL membranes; MICE; PROBABILITY theory; REGENERATION (Biology); RESEARCH funding; SCANNING electron microscopy; SPECTROPHOTOMETRY; STATISTICS; TOXICITY testing; VISCOSITY; PLANT extracts; POLYSTYRENE; DATA analysis; DATA analysis software; DESCRIPTIVE statistics; TENSILE strength; IN vitro studies; ONE-way analysis of variance; EQUIPMENT &; supplies; PREVENTION
- Publication
BioMed Research International, 2016, Vol 2016, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2016/3640182