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- Title
Comparison of the Hemagglutination Inhibition Test and IgG ELISA in Categorizing Primary and Secondary Dengue Infections Based on the Plaque Reduction Neutralization Test.
- Authors
Lukman, Nurhayati; Salim, Gustiani; Kosasih, Herman; Susanto, Nugroho Harry; Parwati, Ida; Fitri, Silvita; Alisjahbana, Bachti; Widjaja, Susana; Williams, Maya
- Abstract
Secondary dengue infection by heterotypic serotypes is associated with severe manifestations of disease, that is, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) has recommended criteria based on the hemagglutination inhibition (HI) test to distinguish between primary and secondary dengue infections. Since the HI test has practical limitations and disadvantages, we evaluated the accuracy of WHO HI criteria and compared it with criteria based on an IgG enzyme-linked immunosorbent assay (ELISA) using a plaque reduction neutralization test (PRNT) as the gold standard. Both WHO HI criteria and IgG ELISA criteria performed strongly (16/16) in determining primary infection. However, to determine secondary infection, the IgG ELISA criteria performed better (72/73) compared to the WHO HI criteria (23/73).
- Subjects
INDONESIA; DIAGNOSIS of fever; IMMUNOGLOBULIN analysis; MIXED infections; BIOLOGICAL assay; COMPARATIVE studies; DENGUE; ENZYME-linked immunosorbent assay; HEMAGGLUTINATION tests; PROBABILITY theory; REGRESSION analysis; WORLD Health Organization; DATA analysis; DATA analysis software; DESCRIPTIVE statistics; NEUTRALIZATION tests; DIAGNOSIS
- Publication
BioMed Research International, 2016, Vol 2016, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2016/5253842