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- Title
Reactive oxygen species production and activation mechanism of the rice NADPH oxidase OsRbohB.
- Authors
Takahashi, Shinya; Kimura, Sachie; Kaya, Hidetaka; Iizuka, Ayako; Wong, Hann Ling; Shimamoto, Ko; Kuchitsu, Kazuyuki
- Abstract
Reactive oxygen species (ROS) produced by plant NADPH oxidases (NOXes) are important in plant innate immunity. The Oryza sativa respiratory burst oxidase homologue B (OsRbohB) gene encodes a NOX the regulatory mechanisms of which are largely unknown. Here, we used a heterologous expression system to demonstrate that OsRbohB shows ROS-producing activity. Treatment with ionomycin, a Ca2+ ionophore, and calyculin A, a protein phosphatase inhibitor, activated ROS-producing activity; it was thus OsRbohB activated by both Ca2+ and protein phosphorylation. Mutation analyses revealed that not only the first EF-hand motif but also the upstream amino-terminal region were necessary for Ca2+-dependent activation, while these regions are not required for phosphorylation-induced ROS production.
- Subjects
REACTIVE oxygen species; OXIDASES; ENZYME activation; CALCIUM ions; RICE; PHOSPHORYLATION; GENETIC mutation; PLANT immunology
- Publication
Journal of Biochemistry, 2012, Vol 152, Issue 1, p37
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvs044