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- Title
Exon 2 deletion splice variant of γ-glutamyl carboxylase causes des-γ-carboxy prothrombin production in hepatocellular carcinoma cell lines
- Authors
Ueda, Naoki; Shiraha, Hidenori; Fujikawa, Tatsuya; Takaoka, Nobuyuki; Nakanishi, Yutaka; Suzuki, Mayumi; Matsuo, Noriyuki; Tanaka, Shigetomi; Nishina, Shin-ichi; Uemura, Masayuki; Takaki, Akinobu; Shiratori, Yasushi; Yamamoto, Kazuhide
- Abstract
Abstract: Using GGCX gene-specific real-time PCR, exon 2 deletion splice variant of vitamin K-dependent γ-glutamyl carboxylase (GGCX) mRNA was identified in HCC cell lines. Expressions of wild type and exon 2 deletion variant of GGCX were analyzed with relevance to DCP production in HCC cell lines. Hep3B, HepG2, HuH1, HuH7, and PLC/PRF/5 produced DCP, while SK-Hep-1, HLE, HLF, and JHH1 produced no detectable level of DCP. DCP-producing cells expressed exon 2 deletion variant of GGCX mRNA and protein, while DCP-negative cells expressed no detectable level of exon 2 deletion variant of GGCX. These results suggest that exon 2 deletion splice variant of GGCX causes dysfunction of GGCX enzyme activity resulting in DCP production in HCC cell lines.
- Subjects
EXONS (Genetics); LIVER cancer; CELL lines; ENZYMES; PROTHROMBIN; GENE expression; POLYMERASE chain reaction
- Publication
Molecular Oncology, 2008, Vol 2, Issue 3, p241
- ISSN
1574-7891
- Publication type
Article
- DOI
10.1016/j.molonc.2008.06.004