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- Title
A Novel In Vitro Assay Correlates Insulin Receptor Autoantibodies With Fasting Insulin in Type B Insulin Resistance.
- Authors
Minich, Waldemar B.; Abel, Brent S.; Schwiebert, Christian; Welsink, Tim; Seemann, Petra; Brown, Rebecca J.; Schomburg, Lutz
- Abstract
Context: Severe insulin resistance (IR) in the presence of insulin receptor autoantibodies (InsR-aAb) is known as type B insulin resistance (TBIR). Considerable progress in therapy has been achieved, but diagnosis and monitoring of InsR-aAb remains a challenge. Objective: This work aimed to establish a robust in vitro method for InsR-Ab quantification. Methods: Longitudinal serum samples from patients with TBIR at the National Institutes of Health were collected. A bridge-assay for InsR-aAb detection was established using recombinant human insulin receptor as bait and detector. Monoclonal antibodies served as positive controls for validation. Results: The novel assay proved sensitive, robust, and passed quality control. The measured InsR-aAb from TBIR patients was associated with disease severity, decreased on treatment, and inhibited insulin signaling in vitro. Titers of InsR-aAb correlated positively to fasting insulin in patients. Conclusion: Quantification of InsR-aAb from serum samples via the novel in vitro assay enables identification of TBIR and monitoring of successful therapy.
- Subjects
INSULIN receptors; AUTOANTIBODIES; INSULIN resistance
- Publication
Journal of Clinical Endocrinology & Metabolism, 2023, Vol 108, Issue 9, p2324
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/clinem/dgad125