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- Title
PREVALENCIA DE LA INFECCIÓN POR Mycobacterium avium SUBESPECIE paratuberculosis EN REBAÑOS DE OVINOS DE DOS MUNICIPIOS DE SAN LUIS POTOSÍ, MÉXICO.
- Authors
Morón-Cedillo, Felipe de Jesús; Cortez-Romero, César; Gallegos-Sánchez, Jaime; Figueroa-Sandoval, Benjamín; Aquino-Pérez, Gildardo; Amante-Orozco, Alejandro
- Abstract
Paratuberculosis (Ptb), also known as Johne's disease, is caused by Mycobacterium avium subspecie paratuberculosis (Map). The disease is contagious in cattle, sheep, goats and other ruminants; in humans it is associated with Crohn's disease. Progressive weight loss is observed in sheep, and in advanced stages, clumped stools and diarrhea. To diagnose this disease there are different tests: one is using the feces, from which an insertion sequence 900 (IS900) Map genome is obtained, making possible the organism identification through a Polymerase Chain Reaction (PCR), and a second one in blood serum through the Agar Gel Immunodiffusion test (AGID). These two techniques were selected to estimate the prevalence of Map. Fecal and blood samples of 211 asymptomatic sheep were analyzed in 32 flocks from 10 communities in Salinas and Villa of Ramos, San Luis Potosi. The Map prevalence per flock between communities with the AGID test ranged from 4.35 to 33.33%, with a mean of 9.48%. The prevalence with the nested PCR test came out with a value varying from 4.26 to 33.33% and a mean of 7.58% between communities. The AGID test detected 20 positive sheep to Map antibodies. The nested PCR detected 16 sheep excreting Map. By using the PCR test as the gold standard, the AGID test had a sensitivity of 81% and a specificity of 96%, with a kappa agreement value of 0.96. Being Map present in the Municipalities of Salinas and Villa de Ramos, San Luis Potosi, it is necessary to take the relevant measures to control and eradicate it, preventing its spread and economic losses in all livestock farms.
- Subjects
SAN Luis Potosi (Mexico); MYCOBACTERIUM avium paratuberculosis; CONTAGIOUS agalactia; DISEASE prevalence; CROHN'S disease; POLYMERASE chain reaction methodology; LIVESTOCK; ECONOMICS
- Publication
Revista Cientifica de la Facultade de Veterinaria, 2013, Vol 23, Issue 4, p293
- ISSN
0798-2259
- Publication type
Article