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- Title
Evaluating the potential of chitosan/poly(vinyl alcohol) membranes as alternative carrier material for proliferation of Vero cells.
- Authors
Sharma, Parul; Mathur, Garima; Goswami, Navendu; Sharma, Sanjeev K.; Dhakate, Sanjay R.; Chand, Subhash; Mathur, Ashwani
- Abstract
Chitosan/poly(vinyl alcohol) (CS/PVA) blend membranes were prepared using the casting method and their physiochemical properties were analyzed using Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and X-ray diffraction (XRD). FTIR and XRD demonstrated possible hydrogen bonds between CS and PVA. The addition of PVA to CS resulted in surface roughness as analyzed by SEM. The CS/PVA blend membrane exhibited high tensile properties (81.62%) and reduced water-holding capacity (53.8%) compared to a pure CS membrane (control). Cell viability and proliferation were assessed via an MTT assay with Vero cell culture. Associated with improved physicochemical properties, the CS/PVA blend membrane promotes cell proliferation of Vero cells with high specific growth rate (0.582 day-1). The results demonstrate that the blending of CS and PVA could significantly alter the surface rugosity, water-holding capacity and improve the mechanical and biological properties of the membrane. Interestingly, this concept can be extended for different anchorage-dependent cell lines, as an alternative carrier material.
- Subjects
CHITOSAN; CELL culture; CELL survival; CELL proliferation; CELL lines; FOURIER transform infrared spectroscopy; SCANNING electron microscopy; DIFFERENTIAL scanning calorimetry
- Publication
e-Polymers, 2015, Vol 15, Issue 4, p237
- ISSN
1618-7229
- Publication type
Article
- DOI
10.1515/epoly-2015-0021