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- Title
Reciprocal Regulation of OmpR and Hfq and Their Regulatory Actions on the Vi Polysaccharide Capsular Antigen in <italic>Salmonella enterica</italic> Serovar Typhi.
- Authors
Zhang, Ying; Xia, Lin; Lin, Liping; Tang, Hao; Osei-Adjei, George; Xu, Shungao; Zhang, Yiquan; Huang, Xinxiang
- Abstract
<italic>Salmonella enterica</italic> serovar Typhi (<italic>S</italic>. Typhi) is the causative agent of human typhoid fever. <italic>S</italic>. Typhi expresses a major virulence determinant called Vi polysaccharide capsular antigen, which is encoded by the <italic>viaB</italic> locus containing 10 consecutive genes including <italic>tviA</italic> and <italic>tviB</italic>. Expression of Vi antigen is regulated by the two-component regulatory system EnvZ/OmpR and the global RNA-binding factor Hfq. In this study, we show that OmpR coordinates with Hfq to regulate the transcription of Vi antigen genes under osmotic stress conditions. OmpR binds to the promoters of <italic>tviA</italic> and its own genes to activate their transcription; however, it positively regulates <italic>tviB</italic> and negatively regulates <italic>hfq</italic> in an indirect manner. Moreover, Hfq reversely inhibits <italic>ompR, tviA</italic> and <italic>tviB</italic>, and positively regulates its own gene expression. Thus, we report of a complex gene regulatory network involving the reciprocal regulation and autoregulation of OmpR and Hfq, and their regulatory actions on Vi polysaccharide capsular antigen genes in <italic>S</italic>. Typhi.
- Subjects
POLYSACCHARIDES; SALMONELLA enterica; TYPHOID fever; RNA-binding proteins; OSMOSIS; GENETIC regulation
- Publication
Current Microbiology, 2018, Vol 75, Issue 6, p773
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-018-1447-7